Its main idea is to move away from quasi-final, off-line product quality assessment to "real time" strategies accompanied by online measurements of critical variables. Here one main point is to easily decide whether a process is stable and reliable in production according to Food and Drug Administration (FDA) standards. Ĭonsidering production processes in biotechnological industry, Process Analytical Technologies (PAT) for monitoring and evaluation of these processes is gaining more and more importance. In industrial production processes, single cell analysis may give high resolution insights into whole cell cultures concerning the cell status of viability, metabolic activity or even productivity. The methods were shown to be suitable tools for process monitoring at single cell level allowing a better process understanding, increasing robustness and forming a firm basis for physiology-based analysis and optimization with the general application for bioprocess development.įlow cytometry was originally established for characterizing and sorting mammalian cells but won recently more and more importance applied at microbial processes, medical applications, dairy industry, alcoholic beverage production and environmental and water systems.
It was possible to distinguish between live, metabolic active, depolarized, dormant, and dead cells and to discriminate between high and low productive cells. The assays established were applied at different stages of a bioprocess where the antibody fragment D1.3 scFv production and secretion by B. Methods were tested regarding their sensitivity by evaluating fluorescence surface density and fluorescent specific concentration in relation to the electronic cell volume. It was possible to establish an appropriate assay to measure the production intensities of single cells revealing certain product secretion dynamics. Here different dyes for viability estimation considering membrane potential (DiOC 2(3), DiBAC 4(3), DiOC 6(3)) and cell integrity (DiBAC 4(3)/PI, Syto9/PI) were successfully evaluated for Bacillus megaterium cell characterization. NOTE: This product is a TRANSMITTER, not a receiver.Single cell analysis for bioprocess monitoring is an important tool to gain deeper insights into particular cell behavior and population dynamics of production processes and can be very useful for discrimination of the real bottleneck between product biosynthesis and secretion, respectively.
#Bluesense off gas sensors Bluetooth
For even MORE creativity, pair with the GOgroove BlueGate Bluetooth Receiver and sync practically ANY two media devices! If you're looking for compatible Bluetooth speakers or headsets, GOgroove has you covered! Be sure to check out our speakers like the GOgroove Bluesync MC or GOgroove Bluesync OR3, and headsets like the AirBand or BlueVIBE DLX.
#Bluesense off gas sensors portable
If you have an audio device that isn't Bluetooth capable, the BlueSense is for you! Just sync with a speaker system or headset to enjoy audio at home, or use a portable Bluetooth speaker & stream while camping, tailgating or at your next party! Plug into an audio receiver & pair with a Bluetooth headset for personal audio. Simply enter device into "Pairing Mode," hold down BlueSense's power button for 5 seconds and you're set! With 10 hours of power on a single charge, the uses are endless! Wide Range Compatibility
Easy SetupīlueSense automatically syncs with your Bluetooth speakers, headsets, & headphones. The GOgroove BlueSense TRM is a portable Bluetooth TRANSMITTER allowing wireless streaming from virtually any audio source! Charge via standard USB port & pair with your favorite Bluetooth-enabled speaker system or headset! BlueSense plugs in to any standard 3.5mm audio-out jack for instant streaming convenience.
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